Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1995-12-22
1997-12-23
Snay, Jeffrey
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 8211, 250368, 2504611, G01N 2164
Patent
active
057004285
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a fluorescence detector for measuring fluorescence in a liquid contained in a tube, comprising a light source for irradiating the liquid with fluorescence-generating radiation, a filter through which fluorescence radiation emitted by the liquid passes, and a detector which functions to detect fluorescence radiation that has passed through the filter and to produce an electric signal corresponding to said fluorescence radiation.
The invention also relates to a carrier device for a cuvette intended for simple, reproducible replacement in a fluorescence detector.
BACKGROUND OF THE INVENTION
Many different makes of apparatus for activating and detecting fluorescence radiation from liquids are commercially available. The liquid is introduced into a cuvette or is allowed to flow past a measuring point, where it is irradiated with UV-light from a light source, while resultant fluorescence radiation is sorted out and passed to an intensity measuring detector. In many instances monochromators are used both for producing radiation and for sorting out fluorescence radiation of a given wave-length. Although it is possible to manufacture such instruments with a high degree of sensitivity and therewith allow The detection of small concentrations, such instruments are unavoidably very expensive. Simpler instruments which operate solely with colour filters are also available, although these instruments are not able to satisfy the same high requirements at present.
SUMMARY OF THE INVENTION
An object of the present invention is to provide a fluorescence detector which is simple and inexpensive in manufacture but which, nevertheless, will provide high detection sensitivity. The detector is primarily intended to be used with through-flowing samples from small chromatography columns or in so-called flow injection systems, although it can also be used with static samples. In the case of the applications for which the invention is primarily intended, it is possible to use relatively broad wavelength bands for both the incident exciting radiation and the captured emitted radiation. This is possible because of the high degree of sensitivity that can be obtained due to the effective use of the light, and because it is possible to use colour filters in the majority of cases, provided that these filters are suitably constructed.
Another object of the invention is to provide a simple and practical exchange possibility for cuvettes and samples mounted in exchangeable cassettes.
These and other objects of the invention are achieved in accordance with the invention with a fluorescence detector having the characteristic features set forth in the claims.
One particularly distinguishing feature of one embodiment of the invention is that the filters used have the form of plates cut from filter material and fulfill two different functions simultaneously, namely a filter function and a light conducting function. In this way, there is achieved both a good filter effect for long wavelengths through the filters and favourable collimation, despite the fact that no lenses are required. However, the filter effect can also be achieved by placing separate filters in the beam paths.
With regard to the carrier device or the cassette according to the invention, one characteristic feature resides in the use of an inner comer in the detector, in the form of a surface from which fluorescence exciting light is emitted towards a cuvette or the like, and a surface which is angled in relation to the first-mentioned surface and through which fluorescence light is taken up for detection, wherein the cuvette or like device is mounted resiliently in the cassette and is pressed in towards the two surfaces to take a fully reproducible position when mounting the cassette.
Similar to known fluorescence detectors, there is used a sample cuvette which consists in a thin tube of transparent material, preferably quartz, through which the examined liquid flows. The quartz tube is mounted advantageously so as to
REFERENCES:
patent: 5304492 (1994-04-01), Klinkhammer
patent: 5460943 (1995-10-01), Hayashi et al.
CMA/Microdialuysis Research AB
Snay Jeffrey
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