Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Casein or caseinate
Patent
1998-04-23
2000-06-27
Longton, Enrique D.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Casein or caseinate
530360, 530350, 530412, 530418, 530419, 530420, 530424, 530422, 435 691, 4352523, 4353201, C07K 114
Patent
active
060808446
ABSTRACT:
The present invention involves a process of purifying and recovering a recombinant protein from a suspension of cells. The process of the present invention involves extracting a recombinant protein from a concentrated suspension of cells using a water-miscible organic solvent, isolating the recombinant protein from the suspension of cells, concentrating the recombinant protein to remove the water-miscible organic solvent, precipitating the recombinant protein using an acid, washing the recombinant protein with the salt or free form of a suitable organic acid and recovering the recombinant protein.
REFERENCES:
patent: 5710044 (1998-01-01), Mukerji et al.
patent: 5739407 (1998-04-01), Bergstrom et al.
Hipp et al. (1952) J. Dairy Sci. 35:272-81.
Hansson et al. (1993) Protein Expression and Purification 4:373-81.
Mulvihill (1989) Developments in Dairy Chem. 4:97-130.
Derwent Publications Ltd., London, GB; AN 91-102657, XP002110124 & DD 284 110 A (Humboldt-Univ. Berlin), Nov. 7, 1990 abstract.
Scopes, R.K.: "Protein Purification--Principles and Practice. Ch.2 (`Making an extract`) & 3 (Separation by precipitation)." 1987, Springer-Verlag, New York XP002109597 p. 26-33; p. 45-62.
Dyr, J.E. et al.: "Separation use for purification of recombinant proteins", Journal of Chromatography B: Biomedical Sciences & Applications, vol. 699, No. 1-2, Oct. 10, 1997, p. 383-401, XP004095005, ISSN: 0378-4347 p. 8, p. 11-col. 2, p. 13-col. 1, p. 14-col. 2, p. 16-col. 1.
Novella, et al.: "Improvement of the extraction of penicillin acylase from Escherichia coli cells by a combined use of chemical methods", Biotechnology and Bioengineering, vol. 44, 1994, pp. 379-382, XP002109997, abstract, p. 2, left-hand col., line 4-7.
Hipp, et al.: "Separation of alpha-, beta- and gamma-casein.", J. Dairy Sci., vol. 35, 1952, XP002109595 (whole document) pp. 272-281.
Mulvihill, D.M.: "Caseins and caseinates: Manufacture" IN: Developments in Dairy Chem., vol. 4, Fox, ed. pp. 97-130, Elsevier Appl. Sci. Publ. London, 1989, XP002109621, p. 97-103, p. 112, paragraph 3, p. 115-119, p. 123-124.
F. M. Ausubel et al., eds., Current Protocols in Molecular Biology, vol. 2, John Wiley & Sons, Inc., (1993), pp. 10.9.2.
Arndt Joseph
Carney Ronald E.
List Julie R.
Schwartz Ellen Marie
Abbott Laboratories
Longton Enrique D.
Pope Lawrense S.
Steele Gregory W.
LandOfFree
Process for the recovery and purification of a recombinant prote does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Process for the recovery and purification of a recombinant prote, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Process for the recovery and purification of a recombinant prote will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1785491