Cell tests for Alzheimer's disease

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530326, 5303879, 5303881, 5303891, G01N 3353, A61K 3800, C07K 1600

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06080582&

ABSTRACT:
The present invention provides methods for the diagnosis of Alzheimer's disease using human cells. Specifically, one method detects differences between potassium channels in cells from Alzheimer's patient and normal donors, and differences in intracellular calcium concentrations between Alzheimer's and normal cells in response to chemicals known to increase intracellular calcium levels. Other methods detect differences between the memory associated GTP binding Cp20 protein levels between Alzheimer's and normal cells.

REFERENCES:
patent: 4925664 (1990-05-01), Jackson et al.
R. Etcheberrigaray et al., Soc. Neurosci Abstract, vol. 18, Oct. 25-30, 1992 "Distinguishing Features of Potassium Channels in Fibroblasts From Alzheimer Aged and Young Donors".
D. Dewar et al., Neurobiol. Aging. vol. 13 (Suppl. 1), Jul. 12-17, 1992 "Multiple Ion Channel Binding Sites are Differentially Altered in Alzheimer's Disease Cortex".
M. Ikeda et al., Brain Res. vol. 567, pp. 51-56 (1991) "Selective Reduction of Iodine-125 Apamin Binding Sites in Alzheimer's Hippocampus: A Quantitative Autoradiographic Study".
R. Etcheberrigaray et al. Proc. Nat'l Ac. Sciences, vol. 89, pp. 7184-7188 (1992) "Classical Conditioning and Protein Kinase C Activation Regulate the Same Single Potassium Channel in Hermissenda Crassicornis Photoreceptors".
B. Sakmann and E. Neker, Ann. Rev. Physiol. vol. 46, pp. 455-472 (1984) "Patch Clamp Techniques For Studing Ionic Channels in Excitable Membranes".
E. Ito et al., Neuroscience Research, vol. 18: Abstract 615 (1993) "A Laboratory Diagnosis Of Alzheimer's Disease With Patch-Clamp and Ca2.sup.+ -imaging techniques".
R. Etcheberrigaray et al., Proceedings Of The National Academy Of Sciences (USA) vol. 90, No. 17. 8209-8213 (1993) "Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease."
E. Ito et al., Proceedings Of The National Academy Of Sciences (USA) vol. 91, No. 2, pp. 534-538 (1994) "Internal Ca2+ mobilization is altered in fibroblasts from patients with Alzheimer disease".
H.M. Huang et al., Neurobiology Of Aging, vol. 12: pp. 469-473 (1994) "Inositol Phosphates and Intracellular Calcium After Bradykinin Stimulation in Fibroblasts From Young, Normal Aged and Alzheimer Donors".
A. Grossmann et al., Neurobiology Of Aging, vol. 14, No. 2: 177-185 (1993) "Intracellular Calcium Response Is Reduced in CD4+ Lymphocytes in Alzheimer's Disease and in Older Persons with Down's Syndrome".
C. Peterson et al., Neurobiology Of Aging, vol. 9, No. 3: 261-266 (1988) "Altered response of Fibroblasts From Aged and Alzheimer Donors to Drugs That Elevate Cytosolic Free Calcium".
Database WPI--Derwent Publications Led. London GB, AN 94-146876 JPA 06,009693 (Elken Kagakukk), Jan. 18, 1994.
A.F. Ghuysen-Itard et al., Gerontology, vol. 39:163-169 (1993) "Loss of Calcium-Homeostatic Mechanisms in Polymorphouclear Leukocytes of Demented and Nondemented Elderly Subjects".
A. Adunsky et al., Journal of Neuroimmunology, vol. 33:167-172 (1991) "Increased Cytosolic Free Calcium In Lymphocytes of Alzheimer Patients".
M. Ikeda et al., Brain Research, vol. 567 51-56 (1991) "Selective Reduction of [.sup.125 I]apamin binding sites in Alzheimer Hippocampus: A Quantitive Autoradiographic Study".
D. Crespo et al., Mechanisms of Aging and Development, vol. 62:223 (1992) "The Influence of Age On Supraoptic Nucleus Neurons Of The Rat: Morphometric And Morphologic Changes" p. 223 only provided.
Alkon et al., J. Neurochem, vol. 51:903 (1988), "Regulation of Hermissenda K+ channels by Cytoplasmic and Membrane-Associated C-Kinase".
J.T. Neary et al., Nature, vol. 293:658 (1981), "Change In A Specific Phosphoprotein band Following Associative Learning In Hermissenda".
T.J. Nelson et al., J.Neurochem, vol. 57:2065 (1991), "Classical Conditioning-Induced Changes in Low-Molecular-Weight GTP-Binding Proteins in Rabbit Hippocampus".
Alkon et al., PNAS (USA) vol. 87:1611 (1990), "Contraction of Neuronal Branching Volume: An Anatomic Correlate of Pavlovian Conditioning".
S. Moshiach et al., Brain Research, vol. 605:298 (1993), "G-Protein Effects On Retrograde Axonal Transport".
T.J. Nelson et al., Science, vol. 247:1479 (1990), "Isolation of G Protein That is Modified by Learning and Reduces Potassium Counts in Hermissenda".
R. Etcheberrigaray et al., Science, vol. 264:276 (1994), "Soluble .beta.-Amyloid Induction of Alzheimer's Phenotype for Human Fibroblast K+ Channels".
Lederhendler, I. Izja, et al. (1990) "Outgrowths From Hermissenda Photoreceptor Somata Are Associated With Activation Of Protein Kinase C" Brain Research 534:195-200.
Lederhendler, I. Izja, et al. (1991) "Outgrowths From Hermissenda Photoreceptor Somata Are Associated With Activation Of Protein Kinase C" Nonmammalian Biochem 114:451, #Abstract 39648Q.
Etcheberrigaray, et al. (1993) "New Implications Of Memory Mechanism For Alzheimer's Disease" Neuroscience Research Communications vol. 13:S7-S10.
Peterson et al. (1986) "Cytosolie Free Calcium and Cell Spreading Decrease in Fibroblasts from Aged and Alzheimer Donors" Proc. Natl. Acad. Sci. vol. 83:7999-8001.
Gunnerson et al. (1991) "Preliminary Characterization of a Novel Alzheimer Disease Associated Protein" vol. 17:1-2 (#271.5).
Morris et al. (1989) The consortium to Establish a Registry for Alzheimer's Disease (CERAD) Neurology 39:1159-1165.
Upstate Biotechnology, Inc., Products for Biological Research. Catalog and Protocols, 1992 pp. 58-61.
Harlow, "Antibodies: A Laboratory Manual" Cold Spring Harbor Laboratory Press 1988, Chapter 6 pp 139-243.

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