Vectors for gene trapping and gene activation

Details Vectors for gene trapping and gene activation Vectors for gene trapping and gene activation

1998-04-08

2000-06-27

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Vector, per se

435325, 435455, 435463, C12N 1563, C12N 1585, C12N 1500

Patent

active

060805765

ABSTRACT:
A novel 3' gene trap cassette is described that does not encode a marker conferring antibiotic resistance and can be used to efficiently trap and identify cellular genes. Vectors incorporating the presently 3' gene trap cassette find particular application in gene discovery, the production of transgenic cells and animals, and gene activation.

REFERENCES:
patent: 4190496 (1980-02-01), Rubenstein et al.
patent: 5449614 (1995-09-01), Danos et al.
patent: 5464764 (1995-11-01), Capecchi et al.
patent: 5521076 (1996-05-01), Mulligan et al.
patent: 5625048 (1997-04-01), Tsien et al.
patent: 5733761 (1998-03-01), Treco et al.
Campbell et al. (Jan. 1997) Theriogenology, vol. 47, 63-72.
Evans et al. (Sep. 1997) TIG, vol. 13 (9), 340-347.
Niwa et al. (1993) J. Biochem., vol. 113, 343-349.
Skarnes et al. (1993) Curr. Opin. Biotech., vol. 4, 684-689.
Yoshida et al., 1995, "A new strategy of gene trapping in ES cells using 3'RACE", Transgenic Research 4:277-287.
Campbell et al., 1997, "Totipotency or Multipotentiality of Cultured Cells: Applications and Progress", Theriogenology 47:63-72.
Chang et al., 1993, "Enrichment of Insertional Mutants Following Retrovirus Gene Trap Selection", Virology 193:737-747.
Evans et al.,1997, "Gene trapping and functional genomics", TIG 13(9):370-374.
Gogos et al., 1997, "Selection for Retroviral Insertions into Regulated Genes", Journal of Virology 71(2): 1644-1650.
Gogos et al., 1996, "Gene Trapping in Differentiating Cell Lines: Regulation of the Lysosomal Protease Cathepsin B in Skeletal Myoblast Growth and Fusion", Journal of Cell Biology 134(4):837-847.
Hicks et al., 1997, "Functional genomics in mice by tagged sequence mutagenesis", Nature Genetics 16:338-344.
Jonsson et al., 1996, "Use of a Promoter-Trap Retrovirus to Identify and Isolate Genes Involved in Differentiation of a Myeloid Progenitor Cell Line In Vitro", Blood 87(5): 1771-1779.
Niwa et al., 1993, "An Efficient Gene-Trap Method Using Poly A Trap Vectors and Characterization of gene-trap events", J. Biochem 113(3):343-349.
Skarnes, 1993, "The Identification of new genes: gene trapping in transgenic mice", Current Opinion in Biotechnology 4:684-689.
Zambrowicz et al., 1997, "Disruption of overlapping transcripts in the ROSA .beta.geo 26 gene trap strain leads to widespread expression of .beta.-galactosidase in mouse embryos and hematopoietic cells", Proc. Natl. Acad. Sci. USA 94:3789-3794.
Cho et al., 1976, "Revertants of Human Cells Transformed by Murine Sarcoma Virus," Science 194:951-953.
Frohman, 1994, "On Beyond Classic RACE (Rapid Amplification of cDNA Ends)," PCR Methods and Applications 4:S40-S58.
Houghten et al., 1991, "Generation and use of synthetic peptide combinatorial libraries of basic research and drug discovery," Nature 354:84-86.
Inoue et al., 1983, "Rat Mutant Cells Showing Temperature Sensitivity for Transformation by Wild-Type Moloney Murine Sarcoma Virus," Virology 125:242-245.
Lam et al., 1991, "A new type of synthetic peptide library for identifying ligand-binding activity," Nature 354:82-84.
Maruyama et al., 1981, "Characterization of Flat Revertant Cells Isolated from Simian Virus 40-Transformed Mouse and Rat Cells Which Contain Multiple Copies of Viral Genomes," J. Virol. 37:1028-1043.
Mathey-Prevot et al., 1984, "Revertants and Partial Transformants of Rat Fibroblasts Infected with Fujinami Sarcoma Virus," J. Virol. 50:325-334.
Norton et al., 1984, "Expression of Kirsten Murine Sarcoma Virus in Transformed Nonproducer and Revertant NIH/3T3 Cells: Evidence for Cell-Mediated Resistance to a Viral Oncogene in Phenotypic Reversion," J. Virol. 50:439-444.
Pestov and Lau, 1994, "Genetic selection of growth-inhibitory sequences in mammalian cells," Proc. Natl. Acad. Sci. U.S.A. 91:12549-12553.
Ryan et al., 1985, "Isolation of a Simian Virus 40 T-Antigen-Positive, Transformation-Resistant Cell Line by Indirect Selection," Mol. Cell. Biol. 5:3577-3582.
Sacks et al., 1979, "Abelson Murine Leukemia Virus-Infected Cell Lines Defective in Transformation," Virology 97:231-240.
Selten et al., 1985, "Proviral activation of the putative oncogene Pim-1 in MuLV induced T-cell lymphomas," EMBO Journal 4(7):1793-1798.
Songyang et al., 1993, "SH2 Domains Recognize Specific Phosphopeptide Sequences," Cell 72: 767-778.
Stephenson et al., 1973, "Characterization of Morphologic Revertants of Murine and Avian Sarcoma Virus-Transformed Cells," J. Virology 11:218-222.
Varmus et al. 1981, "Retroviruses as Mutagens: Insertion and Excision of a Nontransforming Provirus Alter Expression of a Resident Transforming Provirus," Cell 25:23-36.
Varmus et al., 1981, "Revertants of an ASV-Transformed Rat Cell Line Have Lost the Complete Provirus or Sustained Mutations in src," Virology 108: 28-46.
Vitaterna et al., 1994, "Mutagenesis and Mapping of a Mouse Gene, Clock, Essential for Circadian Behavior," Science 264:719-725.
Wilson et al., 1986, "A Frameshift at a Mutational Hotspot in the Polyoma Virus Early Region Generates Two New Proteins That Define T-Antigen Functional Domains," Cell 44: 477-487.

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