Expression of biologically active PDGF analogs in eucaryotic cel

Details Expression of biologically active PDGF analogs in eucaryotic cel Expression of biologically active PDGF analogs in eucaryotic cel

1985-02-25

1989-01-31

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435 70, 435 91, 435255, 435320, 935 13, 935 28, 935 37, 536 27, C12N 1500, C12N 500, C12P 2100, C12P 1900

Patent

active

048015423

ABSTRACT:
Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the expression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sarcoma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. In particular, DNA sequences encoding polypeptides substantially homologous to the B chain of PDGF are preferred. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the host cell. Mitogenic activity is one of the biological activities possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.

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