Method for performing fluorescent protein binding assay employin

Details Method for performing fluorescent protein binding assay employin Method for performing fluorescent protein binding assay employin

1984-10-23

1986-05-13

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436528, 436531, 436532, 436546, 436800, 436537, 530406, 530307, G01N 33543, G01N 33545, G01N 33549, G01N 33533

Patent

active

045886970

ABSTRACT:
Fluorescent antigen conjugates are provided comprising antigens covalantly bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.

REFERENCES:
patent: 4174384 (1979-11-01), Ullman et al.
patent: 4331590 (1982-05-01), Bocuslaski et al.
patent: 4351760 (1982-09-01), Khanna et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4476228 (1984-10-01), Huchzermeier et al.
patent: 4476229 (1984-10-01), Find et al.
patent: 4481136 (1984-11-01), Khanna et al.

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