Method, test element and test kit for semi-quantitative detectio

Details Method, test element and test kit for semi-quantitative detectio Method, test element and test kit for semi-quantitative detectio

1994-03-31

1997-01-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

055915809

ABSTRACT:
A target nucleic acid can be detected in a semi-quantitative fashion by passing it over detection deposits in a test element. The detection deposits include particles affixed to the test element, some of which particles have capture probe attached thereto, and other particles having no capture probe. The deposits have varying amounts of capture probe so that the signal obtained when the target nucleic acid is captured thereon can be semi-quantitatively correlated to the amount of target nucleic acid in the specimen. This method of detection can be used in nucleic acid hybridization assays or following amplification methods, including polymerase chain reaction.

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patent: 5273882 (1993-12-01), Snitman et al.
patent: 5374524 (1994-12-01), Miller
patent: 5380489 (1995-01-01), Sutton et al.
patent: 5387510 (1995-02-01), Wu

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