Method for determining a presence or absence of a nonparaffinoph

Details Method for determining a presence or absence of a nonparaffinoph Method for determining a presence or absence of a nonparaffinoph

1997-11-13

1998-12-08

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 34, 435 4, 435 30, 435 36, 435 42, 435975, 436 63, C12Q 102, C12Q 104, C12Q 124, C12Q 100

Patent

active

058467602

ABSTRACT:
The method of the invention involves providing a first receptacle and a second receptacle. The first receptacle contains a sterile aqueous broth and the second receptacle contains an aqueous broth including a carbon source. The method then includes placing into the first receptacle a first support surface having a paraffin wax coating thereon and placing into the second receptacle a second support surface having a hydrophobic material coating thereon. A body specimen, such as sputum, is then introduced into each of the first and second receptacles. The presence of a nonparaffinophilic hydrophobic microorganism in the body specimen is determined by observing (i) a lack of microorganism growth on the paraffin coated material of the first support surface and (ii) a presence of microorganism growth on the hydrophobic material coating of the second support surface.
An associated kit is also disclosed.

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