Thermostable luciferase of firefly, thermostable luciferase gene

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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435 691, 435 712, 4352523, 43525233, 4353201, 4351723, 4351721, 536 232, 935 9, 935 14, 935 29, 935 56, 935 72, 935 73, C12N 902, C12N 1553

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052292851

ABSTRACT:
The present invention relates to thermostable luciferase of firefly wherein an amino acid at the 217-position of the amino acid sequence of wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of thermostable firefly luciferase comprising use of said vector.

REFERENCES:
Abstract, Ester et al., Biolumin., Chemilumin. Proc. Int. Symp., 6th Meeting Date 1990, Chichester, UK, "Engineering of a bacterial luciferase alpha-beta fusion protein with enhanced activity at 37.degree. C. in Escherichia coli".

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