Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1992-06-23
1993-07-20
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 691, 435 712, 4352523, 43525233, 4353201, 4351723, 4351721, 536 232, 935 9, 935 14, 935 29, 935 56, 935 72, 935 73, C12N 902, C12N 1553
Patent
active
052292851
ABSTRACT:
The present invention relates to thermostable luciferase of firefly wherein an amino acid at the 217-position of the amino acid sequence of wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of thermostable firefly luciferase comprising use of said vector.
REFERENCES:
Abstract, Ester et al., Biolumin., Chemilumin. Proc. Int. Symp., 6th Meeting Date 1990, Chichester, UK, "Engineering of a bacterial luciferase alpha-beta fusion protein with enhanced activity at 37.degree. C. in Escherichia coli".
Kajiyama Naoki
Nakano Eiichi
Kikkoman Corporation
Prouty Rebecca
Wax Robert A.
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