Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-01-26
1998-12-08
Prats, Francisco
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435975, 935 77, 536 254, C12Q 168, C12P 1934, C12N 1500, C07H 2100
Patent
active
058467165
ABSTRACT:
This invention relates to an improvement of the procedure for amplifying a target nucleotide sequence by using an effective amount of a glycine-based osmolyte in the reaction mixture of an amplification procedure. It has been found that the use of a glycine-based osmolyte reduces the appearance of stutter bands in the amplification product allowing for easier detection of the target nucleotide sequence. For example, detection of the target trinucleotide repeat sequence, indicative of Huntington's Disease, is made clearer with the use of a glycine-based osmolyte.
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Genzyme Corporation
Prats Francisco
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