Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-07-17
1991-10-01
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4351722, 530387, 530809, 935 95, 935103, C12N 500, C12N 1500, C07K 1514
Patent
active
050533363
ABSTRACT:
Monoclonal antibodies are described which have specific affinities for halogenated nucleoside analogs and are preferentially selective for one particular halogen. Such antibodies, when incorporated into immunochemical reagents, may be used to identify and independently quantify the cell division character of more than one population or subpopulation in flow cytometric measurements. Independent assessment of division activity in cell sub-populations facilitates selection of appropriate time and dose for administration of anti-proliferative agents. The hybridomas which secrete halogen selective antibodies and the method of making them are described.
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patent: 4812394 (1989-03-01), Dolbeare et al.
Halloran et al., "The Preparation of Nucleotide-Protein Conjugater: Carbodiimides as Coupling Agents", J. Immunology; vol. 99, pp. 373-377.
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Edward S. Golieb, "The Cellular Basis of the Imune Response: An Approach to Immunology", 1977, Sinauer Associates, Inc., Sunderland, Mass., pp. 248-252.
Campana, D., E. Coustan-Smith and G. Janossy, "Double and Triple Staining Methods for Studying the Proliferative Activity of Human B and T Lymphoid Cells", J. Immunological Methods 107: 79-88 (1988).
Kuo, W.-L., F. Dolbeare, M. Pallavicini and J. W. Gray "Cell Kinetic Studies of the BN Myelocytic Leukemia Labeled with Bromo- and Iodo-deoxyuridine Before and After Multiple Treatment of 1-B-D-Arabinofuranosycytosine", Lawrence Livermore National Laboratory Report No. UCRL-101039, Abstract.
Dolbeare, F., W.-L. Kuo, W. Beisker, M. Vanderlaan and J. W. Gray, "Current Methods in Using Monoclonal Antibodies to do Halopyrimidine/DNA Analysis by Flow Cytometry", Lawrence Livermore National Laboratory Report No. UCRL-100510, Abstract.
Stanker Larry H.
Vanderlaan Martin
Watkins Bruce E.
Hackett Nora A.
Hoffer Florina B.
Kepplinger Esther L.
Lauder Leona L.
Regents of the University of California
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