Method of detecting microbes utilizing chemiluminescent compound

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 19, 435 21, 536 181, C07C 1368, C09K 300, C07D30504, C12Q 110

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052234029

ABSTRACT:
A method of detecting, identifying, and enumerating microbes in biological and non-biological samples includes the steps of combining the sample with a triggerable chemiluminescent compound specifically susceptible to the initiation of chemiluminescent decomposition by at least one microbial enzyme in the sample and detecting and integrating light emission over an extended period of time as an indication of the presence, identification, or enumeration of the microbes in the sample.

REFERENCES:
patent: 3564588 (1971-02-01), Soli
patent: 3959081 (1976-05-01), Witz et al.
patent: 4925789 (1990-05-01), Edberg
patent: 4931223 (1990-06-01), Bronstein
patent: 4952707 (1990-08-01), Edwards et al.
patent: 4959182 (1990-09-01), Schaap
patent: 4962192 (1990-10-01), Schaap
patent: 4978614 (1990-12-01), Bronstein
Rawn, 1983, Biochemistry p. 287, Harper & Row, New York.
Trepeta et al. (Feb. 1984) Methylumbelliferyl-.beta.-D-Glucuronide-Based Medium for Rapid Isolation and Identification of E. coli J. Clin Microbiol 19:172-4.
Edberg et al. (Sep. 1986) Comparison of .beta.-Glucuronidase-Based Substrate Systems for Identification of E. coli J. Clin Chem 24:368-371.
Edberg et al. (Jun. 1988) National Field Evaluation of Defined Substrate Method for the Simultaneous Enumeration of Total Coliforms and E. coli From Drinking Water: Comparison with the Standard Multiple Tube Fermentation Method. Appl. Environ. Microbiol 54:1595-1601.
Berg et al (Aug. 1988) Rapid Detection of Total and Fecal Coliforms in Water by Enzymatic Hydrolysis . . . Appl Env Micro 54:2118-2122.
Schaap et al. (Sep. 1989) Chemiluminescent Substrates for Alkaline Phosphatase: Application to Ultrasensitive Enzyme-Linked) Immunoassays and DNA Probes Clin. Chem 35:1863-4.
Schaap et al. (1987) Chem. and Enzymatic triggering of 1,2-dioxetanes 3: Alkaline phosphatase catalyzed chemiluminescence from aryl phosphate-substituted dioxetane. Tetrahedron Lett 28:1159-62.

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