Hybridization method and probe

Details Hybridization method and probe Hybridization method and probe

1989-04-17

1991-10-01

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435803, 435810, 436501, 530358, 530395, 935 78, C12Q 168, G01N 33566, A61K 3708, C07K 300

Patent

active

050533266

ABSTRACT:
In order to carry out hybridization reactions by forming a complex between a polynucleotide and a detectable substance and using the complex thus formed for hybridization reactions, a single strand polynucleotide is reacted with a positively charged nucleic acid binding detectable protein or polyamine in solution and the resultant complex is covalently bound with a cross-linking agent and the polynucleotide compound obtained is used as a marker in hybridization experiments in the elucidation of complementary sequences in foreign polynucleotides. The protein may be an enzyme such as horseradish peroxidase modified by a polyamine e.g. a polyethylene imine to provide a positively charged region. Assay sensitivity may be enhanced by performing the hybridization in the presence of about 6% (w/v) of a polyethylene glycol.

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Alberts, B. et al., Molecular Biology of the Cell, Garland Pub. Inc., N.Y., 1983, p. 389.
Kennell, D. E., In: Progress in Nucleic Acid Research and Molecular Biology (Davison, J. N. et al., ed.), vol. 11, 1971, Academic Press, N.Y., pp. 259-261.

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