Modified avidin and streptavidin and methods of use thereof

Details Modified avidin and streptavidin and methods of use thereof Modified avidin and streptavidin and methods of use thereof

1995-06-07

1998-12-08

Achutamurthy, Ponnathapura

Drug, bio-affecting and body treating compositions

Conjugate or complex of monoclonal or polyclonal antibody,...

424 149, 424 91, 424 934, 424 935, 4241791, 4241831, 530395, 530402, 530403, 530406, A61K 39395, A61K 3944, C07K 1700

Patent

active

058465375

ABSTRACT:
The present invention provides modified avidin and streptavidin compounds that have suitable blood clearance kinetics for use in a two-step approach to deliver a molecule to a target site. In particular, to hasten SA's blood clearance carbohydrate moieties are covalently bonded to SA. To prolong Avid's blood clearance, Avid is deglycosylated and/or neutralized by alkylation of its lysine amino acids. In a two-step approach, biotinylated compounds are used to deliver radionuclides, cytotoxic drugs, MRI agents, fluorochromes and other agents suitable for imaging and therapy to target-bound modified streptavidin or avidin conjugated antibodies or other targeting agents.

REFERENCES:
patent: 4401647 (1983-08-01), Krohn et al.
patent: 4863713 (1989-09-01), Goodwin et al.
patent: 5171578 (1992-12-01), Bally et al.
patent: 5326778 (1994-07-01), Rosebrough
Marshall, et al. (1995) "Galactosylated Streptavidin for Improved Clearance of Biotinylated Intact and F (ab').sub.2 Fragments of an Anti-Tumour Antibody" British Journal of Cancer 71:18-24.
Marshall, et al. (1996) "Polyethylene Glycol Modification of a Galactosylated Streptavidin Clearing Agent: Effects on Immunogenicity and Clearance of a Biotinylated Anti-Tumour Antibody" British Journal of Cancer 73 (5) :565-572.
Hiller et al. (1987) "Bioton binding to avidin" Biochem. J. 248:167-171.
Hnatowich et al. (1987) "Investigations of avidin and biotin for imaging applications" J. Nucl. Med. 28:1294-1302.
Kalofonos et al. (1990) "Imaging of tumor in patients with indium-111-labeled biotin and streptavidin-conjugated antibodies: preliminary communication" J. Nucl. Med. 31:1791-1796.
Kang et al. (1994) "Use of neutral avidin improves pharmocokinetics and brain delivery of biotin bound to an avidin-monoclonal antibody conjugate" J. Pharmacol. Exp. Ther. 269:344-350.
Mattes (1987) "Biodistribution of antibodies after intraperitoneal or intravenous injection and effect of carbohydrate modifications" JNCI 79:855-863.
Ong et al. (1991) "Galactose-conjugated antibodies in cancer therapy: properties and principles of action" Cancer Research 51:1619-1626.
Rosebrough (1993) "Plasma stability and pharmacokinetics of radiolabeled deferoxamine-biotin derivatives" J. Pharmacol. Exp.Ther. 265:408-415.
Rosebrough (1993) "Pharmacokinetics and biodistribution of radiolabeled avidin, streptavidin and biotin" Nucl. Med. Biol. 20:663-668.
Wilchek et al. (1993) "Avidin-biotin immobilisation systems" in Application of Immobilized Molecules, Sletyr et al., eds., Springer Verlag, NY, 51-60.
Winkelhake et al. (1976) "Aglycosylantibody" J. Biol. Chem. 251:1074.

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