Method of preparing an enzyme-labeled ligand for use in specific

Details Method of preparing an enzyme-labeled ligand for use in specific Method of preparing an enzyme-labeled ligand for use in specific

1981-03-16

1983-03-08

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 7, 435189, 435190, C12N 996, G01N 3354, C12N 904, C12N 902

Patent

active

043761658

ABSTRACT:
A method of preparing an enzyme-labeled ligand for use in specific binding assays for determining said ligand or a specific binding partner thereof, by covalently coupling said ligand or a binding analog thereof to an organic prosthetic group and combining the resulting coupled product with an apoenzyme to form a conjugated enzyme complex with said prosthetic group in said coupled product. Preferably, the conjugated enzyme complex in the resulting labeled conjugate exhibits holoenzyme activity which is affected by binding of said labeled conjugate by a specific binding partner of said ligand. Preferred prosthetic groups are flavin mononucleotide, heme, and, especially, flavin adenine dinucleotide, with preferred apoenzymes being apoperoxidase and, especially, apoglucose oxidase.

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