Monoclonal antibody specific for DNA.RNA hybrids

Details Monoclonal antibody specific for DNA.RNA hybrids Monoclonal antibody specific for DNA.RNA hybrids

1985-08-26

1989-05-23

Griffin, Ronald W.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 6, 435 7, 435 68, 530387, 935 78, 436548, C12N 500, C07K 1506, C12Q 168, C12P 2100

Patent

active

048330841

ABSTRACT:
A monoclonal antibody specific for DNA.multidot.RNA duplexes, particularly DNA.multidot.RNA heteropolymer duplexes, characterized by having cross-reactivity for binding to single- or double-stranded DNA or RNA as measured by competitive immunoassay of less than about 1:1000, and preferably less than 1:10,000, and an affinity for DNA.multidot.RNA heteropolymer duplexes greater than 10.sup.9 L/mole. The monoclonal antibody is prepared by conventional somatic cell hybridization techniques wherein the host animal is preferably immunized with an immunogen comprising a random DNA.multidot.RNA heteropolymer. The antibody, particularly in a labeled form, is useful in the specific detection of DNA.multidot.RNA duplexes in a test medium such as a nucleic acid hybridization assay mixture.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4376110 (1983-03-01), David et al.
patent: 4732847 (1988-03-01), Stuart et al.
patent: 4743535 (1988-05-01), Carrico
Stuart, W. D. et al., Proc. Natl. Acad. Sci. U.S.A., vol. 78, No. 6 1981, pp. 3751-3754.
St. Groth, S. In Handbook of Monoclonal Antibodies (Ferrone, S. et al. ed.) Noyes Publications, New Jersey, 1985, p. 7.

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