Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1981-07-16
1983-09-13
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435240, 435241, 435317, 424177, 435948, 435 41, C12P 2100, C12N 500, C12N 502, C12N 100, A61K 3700, C12R 191, C12P 100
Patent
active
044042809
ABSTRACT:
A process for producing murine IL-2 from malignant neoplastic cells which are incapable of IL-2 production by mitogen stimulation alone includes culturing murine leukemia or lymphoma cells in vitro in a protein-containing medium supplemented with various additives. A T cell mitogen and IL-1 are added to the culture medium as co-stimulants inducing the production of a supernate which contains IL-2. After a period of time, the supernate is collected and then assayed for IL-2 activity. Also in the present invention, IL-1 is utilized as a co-stimulant together with a suboptimum concentration of a T cell mitogen to induce IL-2 production in cell lines capable of generating IL-2 by mitogen stimulation alone. The use of IL-1 in these instances reduces the quantity of mitogen required to produce maximum levels of IL-2.
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Martinell James
Tanenholtz Alvin E.
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