Pseudo telecentric optical design for flow cytometric blood cell

Details Pseudo telecentric optical design for flow cytometric blood cell Pseudo telecentric optical design for flow cytometric blood cell

1995-07-28

1997-05-20

Rosenberger, Richard A.

Optics: measuring and testing

For optical fiber or waveguide inspection

356339, G01N 2153

Patent

active

056317301

ABSTRACT:
A pseudo telecentric flow cytometric optical system for the simultaneous detection of several characteristics of particles suspended in a flowing medium. The system is made up of a flowcell through which the particles pass substantially one particle at a time, an optical system for directing light from a light source onto the flowing particles in the flowcell, a side angle optical collection system for receiving light from the flowing particles and for directing the light to one or more of a first set of detectors, and a forward angle collection system for receiving light from the flowing particles and for directing the light to one or more of a second set of detectors. The side angle optical collection system can include a condenser lens for directing light toward the first set of detectors with an exit pupil of the condenser lens located at the back focal plane of the condenser lens; a photosensitive surface of one or more of the first set of detectors is located at conjugate points of the back focal plane of the condenser lens such that an image of the exit pupil is positioned at the photosensitive surface of one or more of the first set of detectors. The forward angle optical collection system can include a collecting lens for directing light toward the second set of detectors where the collecting lens exit pupil is located in the back focal plane of the collecting lens and a photosensitive surface of one or more of the second set of detectors is located at the back focal plane of the collecting lens.

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Jacobs, Donald H. "Fundamentals of Optical Engineering", first edition, 1943. McGraw-Hill Book Company, Inc. pp. 52-53.

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