Oligonucleotides containing base-free linking groups with photoa

Details Oligonucleotides containing base-free linking groups with photoa Oligonucleotides containing base-free linking groups with photoa

1995-06-07

1998-06-16

Sisson, Bradley L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2432, 536 2433, 435 6, 435 911, 435 912, 435810, 935 6, 935 8, 935 76, 935 77, C07H 2102, C12Q 168, C12N 1500

Patent

active

057672598

ABSTRACT:
Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.

REFERENCES:
patent: 4851330 (1989-07-01), Kohne
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 5082934 (1992-01-01), Saba et al.
patent: 5185243 (1993-02-01), Ullman et al.
patent: 5219734 (1993-06-01), Royer et al.
patent: 5449602 (1995-09-01), Royer et al.

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