Method of determining concentration of luteinizing hormone in bo

Details Method of determining concentration of luteinizing hormone in bo Method of determining concentration of luteinizing hormone in bo

1975-04-17

1976-11-09

Thomas, Jr., James O.

Drug, bio-affecting and body treating compositions

Identification or warning feature

Taste or smell or chemical irritation to the eye, nose, or...

424100, 424105, A61K 3900, G01N 3100, G01N 3316

Patent

active

039911748

ABSTRACT:
The invention relates to a method of determining the concentration of luteinizing hormone (hereinafter referred to as "LH") in the body fluid of a woman and thereby determining the day when said woman ovulates, as well as to compositions for effecting this determination. The composition comprises human chorionic gonadotropin (hereinafter referred to as "HCG") coupled to red blood cells by means of glutaraldehyde as coupling agent therebetween, which composition in carrying out the test is mixed with the body fluid of the woman and an anti-HCG serum in an amount adjusted to react with approximately 50 international units (I.U.) of HCG per liter of the body fluid, generally urine, so that agglutination occurs when LH equivalent to less than 50 I.U. of HCG/L is present and no agglutination when at least 50 I.U. HCG/L is present. The day of highest concentration of LG equivalent to HCG indicating the day of ovulation.

REFERENCES:
patent: 3553310 (1971-01-01), Csizmas
patent: 3565987 (1971-02-01), Schuurs
Schuurs, Acta Endocrinnol. Suppl., 1969, No. 142, pp. 95-112.
Echt, Chem. Abs., vol. 66, 1967, No. 52591a.
Chem. Abs., vol. 72, 1970, Nos. 51320y, 51321z, 51322a, 51324c.
Reiss, Chem. Abs., vol. 67, 1967, No. 9790y.

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