Hybrid protein C

Details Hybrid protein C Hybrid protein C

1994-10-05

1998-06-16

Ziska, Suzanne E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351723, 435 691, 536 231, 536 232, C12N 1500, C12N 500, C12P 2106, C07H 2102

Patent

active

057669210

ABSTRACT:
Human protein C molecules are modified to provide increased resistance to inactivation by human plasma factors while retaining substantially the biological activity of human protein C. The modifications are generally to the heavy chain of protein C, which chain may be substituted with a protein C heavy chain of non-human origin, such as bovine, yielding a chimeric protein C molecule. The human protein C heavy chain may also be modified to be human-like, in that at least one amino acid from a non-human sequence may be substituted for the corresponding residue(s) of the human sequence, thereby allowing the molecule to retain substantially human characteristics yet having increased resistance to inactivation. Also included are methods for producing the modified protein C molecules and pharmaceutical compositions thereof. The modified molecules, having an increased half-life in human plasma, are particularly useful for treating coagulation-related disorders, such as protein C deficiency or thrombosis, or for promoting fibrinolysis in a patient.

REFERENCES:
patent: 4711848 (1987-12-01), Insely et al.
patent: 4775624 (1988-10-01), Bang et al.
patent: 4902614 (1990-02-01), Wakabayashi et al.
patent: 5077024 (1991-12-01), Brake et al.
Creighton, Prog. Biophys. Molec. Biol. 33:231-297 (1978).
Brot et al., Arch. Biochem. Biophys. 223:221-281 (1983).
Wilkinson, Prac. Protein Chem., (ed.) A. Darbre, pp. 121-148, (1986).
Heeb et al., J. Biol. Chem. 263:1163-1161 (1988).
Yan and Grinnell, Fed. Proc. 46:2243 (1987).
Yan et al., FASEB J. 2:A1412 (1988).
Grinnell et al., Bio/Technology 5:1189-1192 (1987).
Ehrlich et al., J. Biol. Chem. 264:14298-14304 (1989).
Thomas et al., Science 241:226-230 (1980).
Marx, Science 235:285-286 (1987).
Bathurst et al., Science 235:248-350 (1987).
Dmochowska et al., Cell 50:573:584 (1987).
Fuller et al., in Leive ed., Microbiology: 1986 273-278.
Taylor et al., J. Clin. Invest. 79:918-925 (1987).
Sakata et al., Proc. Natl. Acad. Sci. USA 82:1121-1125 (1985).
van Hinsbergh et al., Blood 65:444-451 (1985).
Comp and Esmon, J. Clin. Invest. 68:1221-1228 (1981).
Foster et al., Proc. Natl. Acad. Sci. USA 81:4766-4770 (1984).
Long et al,. Proc. Natl. Acad. Sci. USA 82:4673-4677 (1985).
Foster et al., Proc. Natl. Acad. Sci. USA 82:4673-4677 (1985).
Beckmann et al., Fed. Proc. 44:1069 (1985).
Ehrlich et al., Blood 70:386a (1987).
Okayama et al., Mol. cell. Biol. 2:161-170 (1982).
Miessner et al., Proc. Natl. Acad. Sci. USA 84:4171-4175 (1987).
Walker et al. Thrombosis Res. 22: 321. 1981.
Long et al. PNAS 81: S653. 1984.
Kisiel et al. Biochemistry 16(26) : 5824. 1977.

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