Method of producing and purifying enzymes

Details Method of producing and purifying enzymes Method of producing and purifying enzymes

1995-01-26

1998-06-16

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 697, 530412, 536 234, C12N 938, C12P 2104, A23J 100, C07H 2104

Patent

active

057669147

ABSTRACT:
A method of producing and purifying an enzyme which comprises selecting a spore forming host organism, preparing a genetic construct consisting of a DNA sequence encoding the desired enzyme and a DNA sequence directing synthesis of the desired enzyme during sporulation, inserting the genetic construct into the host organism, culturing the transformed host organism under sporulating conditions to obtain host organism spores with the enzyme integrally associated to the spores, and then treating the host organism and enzyme combination to remove any impurities, if necessary. The free enzyme can be obtained by cleaving the connection between the host organism and the enzyme. The combination of the enzyme and host organism is both a stabilized and an immobilized enzyme preparation.

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