Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1999-01-20
2000-11-28
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 41, 435177, 435182, 4352625, 210601, C12N 1110, C12N 1102, C12N 1104, C12P 100, C02F 300
Patent
active
061534161
ABSTRACT:
Microorganisms or enzymes immobilized in beads are prepared using a combination of calcium alginate, polyethylene glycol (PEG) and polyethylene imide (PEI). An aqueous solution containing 1-14% by weight each of calcium alginate, PEG and PEI is combined with a concentrated solution of a microorganism or an enzyme to form a mixture. The mixture is combined with an aqueous solution of 4-8% w/v CaCl.sub.2 to form spherical beads that are allowed to remain in the solution for 3-4 hours. Thereafter, the beads are rinsed with water for 2-3 minutes, transferred to water in a mixer, and stirred with a magnet for 6-9 hours. The resultant beads containing the microorganism or enzyme can be used for removing inorganic nitrogen and organic carbon in waste water, or in processes for making biochemical products.
REFERENCES:
patent: 4996150 (1991-02-01), Joung et al.
patent: 5175093 (1992-12-01), Seifert
Suhaila, et al. Biotechnology Letters, vol. 4, No. 9, 1982, pp. 611-614.
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