Method of determining melanoma micrometastasis using tyrosinase

Details Method of determining melanoma micrometastasis using tyrosinase Method of determining melanoma micrometastasis using tyrosinase

1999-02-05

2000-11-28

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435912, 435 9151, 536 2431, 536 2433, C12Q 168

Patent

active

061533882

ABSTRACT:
A highly sensitive method to detect melanoma micrometastasis by examining lymph nodes for the presence of tyrosinase messenger RNA. In a preferred mode, this is accomplished using the combination of reverse transcription and double round polymerase chain reaction (RT-PCR). The amplified samples are examined on a 2% agarose gel and tyrosinase is seen as a 207 base pair fragment. The lymph nodes examined are determined using pre- and intra- operative node mapping.

REFERENCES:
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Morton et al. Arch Surgery. 127:392-399, Apr. 1992.
Van der Velde-Zimmermann et al. American Journal of Pathology. 149: 759-764, Sep. 1996.

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