Grafts made from amniotic membrane; methods of separating, prese

Details Grafts made from amniotic membrane; methods of separating, prese Grafts made from amniotic membrane; methods of separating, prese

1998-02-20

2000-11-28

Milliw, V.

Surgery

Miscellaneous

Methods

623 4, A61B 1900

Patent

active

061521426

ABSTRACT:
A method for making, storing and using a surgical graft from human amniotic membrane; the resulting graft; and the storage solution. The amniotic membrane is obtained from human placenta, from which the chorion has been separated. Sheets of the amniotic membrane are cut to size and mounted on filter paper. The cells of the amniotic membrane are killed, preferably while being frozen and thawed in the storage solution. The storage solution comprises a culture medium and a hyperosmotic agent, wherein the hydration of the amniotic membrane is maintained. The membrane can be impregnated with therapeutic agents, prior to storage, for use in post surgical healing or other therapies.

REFERENCES:
Badawy, et al. "Evaluation of Tissue Healing and Adhesion Formation After an Intraabdominal Amniotic Membrane Graft in the Rat," 34 J. Reproductive Med. 198 (1989).
Yokomori, et al., "Advantages and Pitfalls of Amnion Inversion Repair for the Treatment of Large Unruptured Omphalocele: Results of 22 Cases," 23 Journal of Pediatric Surgery 882 (1992).
Sorsby, et al., "Further Experience with Amniotic Membrane Grafts in Caustic Burns of the Eye," 31 Br. J. Opthamology 409-18 (1947).
Sorsby and Symons, "Amniotic Membrane Grafts in Caustic Burns of the Eye (Burns of the Second Degree)," 30 Br. J. Opthamology 337-345 (1946).

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