Heterogeneous immunoassay process

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 793, 435 794, 435962, 435967, 435970, 435973, 436544, 436546, 436805, 436810, 436518, G01N 33543

Patent

active

053066227

ABSTRACT:
The invention teaches a method for determining an analyte in a liquid sample via a heterogeneous assay. The sample is contacted to a carrier material which contains, in addition to one of a labelled analyte analogous substance or a labelled analyte specific substance, a detectable component which does not influence immunological reactions occurring during the course of the assay. A solid phase bound component, which is either an insufficient amount of analyte specific substance or an excess of analyte relative to analyte in the sample is contacted to the sample so as to accomplish formation of solid phase bound complexes. After separation of liquid and solid phase, label is measured in one of these, and the detectable substance is measured in the liquid. Correlation of the values obtained leads to determination of the analyte. Also described is a reagent used in the described method.

REFERENCES:
patent: 4387164 (1983-06-01), Hevey et al.
patent: 4540659 (1985-09-01), Litman et al.
patent: 4803170 (1989-02-01), Stanton et al.
ALDRICH Chemical Company 1986 Catalog p. 24.

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