Method for examination of hormone-dependent tumors

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 29, 435375, C12Q 100, C12Q 102

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059142278

ABSTRACT:
The invention relates to an examination method of hormone-dependent tissue, more particularly to a method for examining hormone-dependent tumors by culturing a tumor tissue in (a) a control culture medium, (b) a culture medium containing a hormone synthetase substrate, and (c) a culture medium containing a hormone synthetase substrate and a hormone synthetase inhibitor, and determining the relative growth ratios of tumor cells of (b) to (a), and (c) to (a) for diagnosing a tumor as being hormone dependent.
This invention provides a method for the simultaneous and easy determination of hormone dependent tumors and the presence of hormone synthetic enzymes. Further, this invention provides a new classification system for tumors and means for indicating medical treatment for a cancer patient.

REFERENCES:
Tuttle et al., "Effect of 5-alpha-reductase inhibition and dexamethasone administration of the growth characteristics and intratumor androgen levels of the human prostate cancer cell line PC-3" The Prostate 24 : 229-36 (1994).
T. Utsumi, "Local Production of Estrogen Via Aromatase and Estrone Sulfatase in Breast Tissue" J. of Japan Surg. Soc. 90(6):919-927 (1987) (English abstract).
T. Yamamoto, et al., "Estrogen Productivity of Endometrium and Endometrial Cancer Tissue; Influence of Aromatase on Proliferation of Endometrial Cancer Cells." J. Steroid Biochem. Molec. Biol. 44(4-6): 463-468 (1993).
The Merck Index, S. Budavari (ed.). #4899 "Invertase" pp. 792-793 (1989).
S. Abraham et al., Lipid Metabolism and Enzyme Activities in Hormone-Dependent and Hormone Independent Mammary Adenocarcinoma in GR MiceJNCI 77(1):233-239 (1986).
J.L. Daechnfeldt et al., "High Affinity Oestradiol Receptors and the Activity of Glucose-6-Phosphate Dehydrogenase and Lactose Synthetase in Mammary Carcinoma of Postmenopausal Women"Br. J. Cancer 31424-428 (1975).
K. Fukuma et al., "Hormone Dependency of Carcinoma of the Human Endometrium" Cancer 51288-294 (1983).
Fukuoka, M., "Antitumor Effects of Aromatase Inhibitors on the Growth of Estrogen Dependent Tumors" Kyoto-furitsu Ika Daigaku Zasshi 101(3):317-333 (1992).
M.D.W.G. Krekels, et al., "Aromatase in the Human Choriocarcimoma JEG-3: Inhibition by R 76 713 in Cultured Cells and in Rumors Grown in Nude Mice" J. Steroid Biochem. Molec. Biol. 38(4):415-422 (1991).
P.E. L.o slashed.nning et al., "Treatment of Breast Cancer with Aromatese Inhibitors--Current Status and Future Prospects" Br. J. Cancer 60: 5-8 (1989).
Y. Osawa et al., "Multiple Functions of Aromatase and the Active Site Structure; Aromatase Its the Placental Estrogen 2-Hydroxylase" J. Steroid Biochem. Molec. Biol. 44(4-6):469-480 (1993).
Pizzini, A. et al., "Aromatase fails to mediate the proliferative effects of adrenal androgens on cultured MCF-7 breast cancer cells," Int. J. Oncol 1 (6):709-712 (1992) (Abstract).
R.J. Santen et al., "Enzymatic Control of Estrogen Production in Human Breast Cancer: Relative Significance of Aromatase Versus Sulfatase Pathways" Ann. New York Acad. of Sci. 464:126-137 (1986).

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