Stabilization of activated galactose oxidase enzyme

Details Stabilization of activated galactose oxidase enzyme Stabilization of activated galactose oxidase enzyme

1978-04-28

1980-09-02

Kaplan, G. L.

Chemistry: electrical and wave energy

Processes and products

435 25, C12Q 154, C12Q 126

Patent

active

042205034

ABSTRACT:
Galactose oxidase enzyme can effectively be used for the determination of a number of different substances if activated and stabilized in accordance with the present invention. Activation is accomplished with a redox agent such as ferricyanide. Stabilization of the activated galactose oxidase is accomplished by addition of cupric ion. Upon stabilization, activated, immobilized galactose oxidase enzymes may be stored and reused for at least 25 days.

REFERENCES:
patent: 3539455 (1970-11-01), Clark
patent: 3886045 (1975-05-01), Meiattini
patent: 3947328 (1976-03-01), Friedenberg et al.
patent: 3979274 (1976-09-01), Newman
patent: 4073713 (1978-02-01), Newman
Taylor et al., "Reprint from Analytical Chemistry", vol. 49, pp. 789-794, May 1977.
Amaral et al., "J. of Biological Chemistry", vol. 238 (1963) pp. 2281-2284.
"Journal of the Am. Chem. Soc.", 98:2 (1976), pp. 626-628.
Clark, "Annals of the New York Academy of Science", 102, (1962) pp. 29-45.
Clark, "Ion and Enzyme Electrodes in Biology and Medicine", Park Press, 1976, pp. 161-172.

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