Factor XIII compositions

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530395, 530412, 530415, 530416, 530417, A61K 3514, A61K 3836, C07K 120, C07K 1439

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active

056124562

ABSTRACT:
Highly purified factor XIII and methods of purifying factor XIII are disclosed. Factor XIII is purified from a biological fluid, such as a cell lysate. The methods provide factor XIII compositions that are greater than 99% pure with respect to contaminating proteins. The methods further provide factor XIII compositions wherein 1% or less of the factor XIII is activated factor XIII.

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Schwartz, et al., J. Biol. Chem. 246, 5851-5854 (1971).
Cook and Holbrook, "The Calcium-Induced Dissociation of Human Plasma Clotting Factor XIII", Biochem. J. 141:79-84, 1974.
Curtis and Lorand, "Fibrin-Stabilizing Factor (Factor XIII)", Methods Enzymol. 45:177-191, 1976.
Separation News, "A Strategy for protein purification", vol. 13.6, Pharmacia, 1986.
Harris et al., "Protein Purification Methods", IRL Press, pp. 51-64, 222-231, 154-157, 1989.
Sofer et al., BioTechniques, pp. 198-203, Nov./Dec. 1983.
Kato et al., Adv. Chromatography, vol. 26, pp. 97-115, 1987.
Scopes, Robert K., "Protein Purification", Second Edition, Springer-Verlag, pp. 178-181 and Chapter 3, 1987.

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