Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-02
1997-03-18
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 32, 935 77, 935 78, C12Q 168, C12Q 118
Patent
active
056121830
ABSTRACT:
A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category or organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
REFERENCES:
Ito et al. (1979) J. Gen. Virol. 43: 103-110.
Kitahara et al. (1976) Antimicrobial Agents And Chemotherapy 9(6): 908-914.
Finegold et al. in Diagnostic Microbial (1982) C. V. Mosby Co. in St. Louis., pp. 532-545.
Arthur Lisa B.
Gen-Probe Incorporated
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