Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-03-30
1991-12-03
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 8, 435 14, 435 18, 435 34, C12Q 168
Patent
active
050700120
ABSTRACT:
Methods and compositions are provided for analyzing in viable cells gene expression regulatory elements and systems. The method can be used to evaluate a transcriptional initiation regulatory system or the presence of a trans acting component. The method finds use in evaluating various transcriptional initiation functional sequences for use in expression of products, for the detection of trans acting agents associated with viruses and for quantitating the presence of infectious viruses. The method comprises introducing an expression cassette encoding an enzyme which has a fluorescent substrate, substantially irreversibly introducing the substrate into the cell host and detecting the formation of fluorescence during a predetermined time interval.
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Fiering Steven
Herzenberg Leonard A.
Nolan Garry P.
Isung Frederick F.
Rowland Bertram I.
The Board of Trustees of the Leland Stanford Junior University
Wax Robert A.
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