Specific binding composition comprising a low pI protein or carb

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 75, 435962, 435975, 436501, 436518, 436808, G01N 33535, G01N 33543, G01N 33569

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active

050513567

ABSTRACT:
A specific binding composition comprises a specific binding species and one or more water-soluble proteins or carbohydrates, substantially none of which has a pI greater than about 5. This composition provides improved sensitivity with lower background in diagnostic tests. Preferably, the species is labeled for detection, for example, with an exzyme. The composition can be included with a dye-providing composition in a diagnostic test kit for use in diagnostic methods.

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A. Voller and D. E. Bidwell, Enzyme Immunoassays, pp. 77-86, Chap. 6 Alternative Immunoassays, Edited by W. P. Collins, John Wiley and Sons, 1985.
Robertson, P. W. et al., Reduction in Non-Specific Binding in Enzyme Immunoassay, Using Casein Hydrolsylate in Serum Diluents, Journ. of Immun. Meth., 26 (1985), pp. 195-197.

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