Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-10-18
1993-07-06
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435968, 436518, 436811, 436815, 436804, G01N 3353, C12Q 100
Patent
active
052253295
ABSTRACT:
Disclosed is a method for the screening of pathological condition in the intestinal tract of an animal or a human being, e.g., intestinal ischemia, necrotizing enterocolitis, inflammatory bowel disease and bowel graft rejection. The method assays elevated levels of lipid binding protein, more particularly intestinal fatty acid binding protein, in a biological sample obtained from the animal or human being. Serum or urine are preferred biological samples. Preferred methods employ an immunological assay such as a radioimmunoassay or an enzyme-linked immunosorbent assay for the detection of intestinal fatty acid binding proteins in the sample.
REFERENCES:
Alpers, D. H., et al., Proc. Natl. Acad. Sci. USA 81: 313-317 (1984).
Ausubel, F. M., et al., eds., Current Protocols In Molecular Biology, John Wiley, New York, 1990 Unit 11.2.2.
Bass, N. M., and Manning J. A., Biochem. Biophys. Res. Commun. 137: 929-935 (1986).
Bradford, M. M., Anal. Biochem. 72: 248-254 (1976).
Dempsey, M. E., et al., Chem. Phys. Lipids 38: 223-237 (9185).
Gordon, J. L., et al. Chem. Phys. Lipids 38: 137-158 (1985).
Grendell, J. H., .sctn.106 in Cecil's Textbook of Medicine, 18th ed., W. B. Saunders Co., Harcourt Brace Jovanovich, Philadelphia, 1988, pp. 760-765.
Lobe, T. E., et al., J. Ped. Surg. 18: 449-452 (1983).
Ockner; R. K., and Manning, A., J. Clin. Invest. 54: 326-338 (1974).
Ockner, R. K., et al., J. Biol. Chem. 257: 7872-7878 (1982).
Sacchettini, J. C., et al., J. Biol. Chem. 263: 5815-5819 (1988).
Sacchettini, J. C., et al., Molec. Cell. Biochem. 98: 81-93 (1990).
Said, B., and Schulz, J. Biol. Chem. 259: 1155-1159 (1984).
Sweetser, D. A., et al., J. Biol. Chem. 262: 16060-16071 (1987).
Varley's Practical Clinical Biochemistry, 6th ed., CRC Press, 1988, pp. 112-113.
Crisman, T. S. et al "Measurement of Rat Heart Fatty Acid Binding Protein by ELISA . . . " 1987 J. Mol. Cell Cardiol. 19: 423-431.
Catty, D. Antibodies Volume I A Practical Approach 1988 IRL Press Ltd., Oxford, England.
Kepplinger Esther L.
Parsons Nancy J.
Yale University
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