Preparation of a water-soluble modified enzyme by covalently bon

Details Preparation of a water-soluble modified enzyme by covalently bon Preparation of a water-soluble modified enzyme by covalently bon

1990-09-13

1992-07-28

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435180, 435183, C12N 996, C12N 1108, C12N 900

Patent

active

051340720

ABSTRACT:
A water-soluble polyurethane-modified enzyme is prepared by reaction of an enzyme in aqueous solution with an aqueous solution of a water-soluble polyurethane pre-polymer/bisulfite adduct at a pH greater than 7 to covalently bond the enzyme to the adduct. Preferably, a ratio of enzyme to pre-polymer/bisulfite adduct of from 1:10 to 10:1 is used.

REFERENCES:
patent: 3984365 (1976-10-01), Lienert et al.
patent: 4094744 (1978-06-01), Hartdegen et al.
patent: 4592957 (1986-06-01), Dahm et al.
patent: 4734439 (1988-03-01), Reischl
Petinaux, Marcel, et al., Chemical Abstracts, vol. 87, No. 6645g, p. 20, 1977.
Abstract: Japanese Patent Application 60-232089 (1985) "Method for Immobilizing Biocatalyst . . . ", Cl. C12N 11/04.

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