Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-09-03
1999-06-08
Wilson, James O.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2541, C07H 106
Patent
active
059105844
ABSTRACT:
Method for isolating plasmid DNA, which comprises the steps of: suspending hydroxyapatite particles in a tris-EDTA buffer solution having a pH of about 7 to about 10, about 10 to about 50 mM of an EDTA alkali metal salt, and about 1 to about 100 mM of tris (hydroxymethyl) aminomethane; adding a solution containing RNA and plasmid DNA, obtained upon bacteriolysis, to the hydroxyapatite particles-containing suspension, thereby adsorbing the RNA onto the hydroxyapatite particles in the suspension; and recovering the plasmid DNA by an ethanol precipitation process and others. The isolation method enables effective separation of the purified plasmid DNA from a solution containing RNA and plasmid DNA in a simplified process, without the use for any special separation device.
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Asahi Kogaku Kogyo Kabushiki Kaisha
Wilson James O.
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