Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-05-20
1999-06-08
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 911, 536 221, 536 243, 536 231, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104
Patent
active
059104090
ABSTRACT:
The present invention provides materials and methods for sensitively and selectively screening biological samples for the presence of Candida albicans, a fungal pathogen of increasing clinical concern. Specifically, nucleic acids, reagents, and primers for DNA amplification are provided that will allow amplification of a 526 base pair oligonucleotide from DNA containing Candida albicans. Methods for using such primers in DNA amplification are also provided.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5075216 (1991-12-01), Innis et al.
patent: 5079352 (1992-01-01), Gelfand et al.
Bodey and Fainstein, Systemic Candidiasis, p. 135 (Eds.) Raven Press, New York (1985).
Holmes, A.R. et al., Yeast-specific DNA probes and their application for the detection of Candida albicans, J. Med. Microbiol., 37:346-351 (1992).
Odds, F.C., In candida and candidosis, (Ed.) Leicester University Press, Leicester, United Kingdom (1989).
Musial, et al., Fungal Infections of the Immunocompromised Host: Clinical and Laboratory Aspects, Clin. Microbiol. Rev. 1:349-364 (1988).
Hopfer, R.L., et al., Detection and differentation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and resriction enzyme analysis, J. Med. Vet. Pharm. 31:65-75 (1993).
Buchman, T.G., et al., Detection of surgical pathogens by in vitro DNA amplification. Part I, Rapid identification of Candida albicans by in vitro amplification of a fungal specific gene. Surgery, 108:338-347 (1990).
Bhattacharjee, The .varies.-aminoadipate pathway for the Biosynthesis of Lysine in Lower Eukaryotes, CRC Critical rev. in Microbiol. 12:131-151 (1985).
Lejohn, Enzyme Regulation, Lysine Pathways and Cell Wall Structures as Indicators of Major Lines of Evolution in Fungi, Nature 231:164-168 (1971).
Vogel, Two Modes of Lysine Synthesis Among Lower Fungi: Evolutionary Significance, Biochim. Biophys, Acta 41:172-174 (1960).
Garrad and Bhattacharjee, Lysine biosynthesis in selected pathogenic fungi: Characterization of Lysine auxotrophs and the cloned LYS1 gene of Candida albicans, J. Bacteriol. 174:7379-7384 (1992).
Fujioka, Chemical mechanism of saccharopine dehydrogenase (NAD, L-lysine forming) as deduced from initial rate pH studies, Arch. Biochem. Biophys. 230:553-559 (1984).
Xuan, et al., Overlapping reading frames at the LYS5 locus in the yeast Yarrowia lipolytica, Mol. Cell. Biol. 10:4795-4806 (1990).
Goshorn, et al., Gene isolation by complementation in Candida albicans and applications to physical and gentic mapping, Infect. And Imm. 60:876-884 (1992).
Nucleic Acid Hybridization, (Eds.) Hames B.D. and Higgens, S.J., IRL Press, Oxford (1985).
R.H. Don, et al., `Touchdown' PCR to circumvent spurious priming during gene amplification. Nucleic Acids Research, 19:4008 (1991).
R. Garrad, et al., Molecular and Functional Analysis of the LYS1 Gene of Candida albicans, Infec. and Imm., 62:11 5027-5031 (1994).
PCR Protocols; a Guide to Methods and Applications, (Eds.) M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White, Academic Press, Inc., New York (1990).
Ye, Z. Et al., Use of .varies.-aminoadipate and lysine as sole nitrogen source by Schizosaccharomyces pombe and selected pathogenic fungi, J. Basic Microbiol. 31 (1991) 2, 149-156.
Kan, V.L., Polymerase Chain Reaction for the Diagnosis of Candidemia, The Journal of Infectious Diseases 1993:168:779-83.
Ford, R.A, et al., Physical and functional characterization of the cloned lsyl+ gene of Schizosaccharomyces pombe, J. Basic Microbiol. 33 (1993) 3, 179-186.
Check, William, Molecular Techniques Shed Light on Fungal Genetics: Genetic analyses are leading researchers to reclassify fungi and better understand fungal pathogens, ASM News, vol. 60, No. 11, 1994, pp. 593-596.
Charles, I.G. et al, Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis, Proc. Natl. Acad. Sci. USA, vol. 86, pp. 3554-3558, May 1989.
The Evolution of Metabolic Function, (Ed.) Mortlock, Robert P., CRC Press, New York, 1992.
DNA Fingerprinting: State of the Science, (Eds.) Pena, S.D.J., Chakraboty, R., Epplen, J.T., Jeffreys, A.J., Birkhauser Verlag basel, Switzerland, 1993.
Ruchel, R., Diagnosis of invasive mycoses in severly immunosuppressed patients, Ann Hematol (1993) 67:1-9.
Cheung, L.L. et al., Development of DNA Probes for Candida albicans, Diagn Microbil Infect Dis, 1988:10:171-179.
Bhattacharjee Jnanendra K.
Bhattacherjee Vasker
Miami University
Tung Joyce
Zitomer Stephanie W.
LandOfFree
Methods and reagents for detecting fungal pathogens in a biologi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and reagents for detecting fungal pathogens in a biologi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and reagents for detecting fungal pathogens in a biologi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1682751