Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1998-04-02
2000-02-08
Mertz, Prema
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 72, 530316, 530329, G01N 33567, C07K 714
Patent
active
06022696&
ABSTRACT:
A unique and novel angiotensin AT4 receptor and AIV ligand system for binding a small N-terminal hexapeptide fragment of Angiotensin II (referred to as AIV, with amino acid sequence Val.sub.1 -Tyr.sub.2 -Ile.sub.3 -His.sub.4 -Pro.sub.5 -Phe.sub.6 ; SEQ. ID. NO. 1) is disclosed. AIV ligand binds saturably, reversibly, specifically, and with high affinity to membrane AT4 receptors in a variety of tissues, including heart, lung, kidney, aorta, brain, liver, and uterus, from many animal species. The AT4 receptor is pharmacologically distinct from classic angiotensin receptors (AT1 or AT2). The system employs AIV or C-terminally truncated or extended AIV-like peptides (e.g., VYIHPFX; SEQ. ID. NO. 8) as the signaling agent, and the AT4 plasma membrane receptor as the detection mechanism. The angiotensin AT4 receptor and receptor fragments (including the receptor binding site domain) are capable of binding a VYIHPF (SEQ. ID. NO. 1) angiotensin AIV N-terminal peptide but not an angiotensin AII or AIII N-terminal peptide, i.e., DRVYIHPF (SEQ. ID. NO. 2) or RVYIHPF (SEQ. ID. NO. 3), respectively. Also disclosed are processes for isolating angiotensin AT4 receptor and AIV angioteninase, identifying angiotensin AIV agonists and antagonists, and constructing diagnostic assays to specifically measure AIV and AI-specific angiotensinase in biological fluids.
REFERENCES:
patent: 3920627 (1975-11-01), Wissman et al.
patent: 5296354 (1994-03-01), Simon et al.
patent: 5464821 (1995-11-01), Stig et al.
patent: 5470753 (1995-11-01), Sepetov et al.
Peach, M.J., "Renin-Angiotensin System: Biochemistry and Mechanisms of Action," Physio. Rev. 57:313-370 (1977).
Johnston, C.I., "Biochemistry and Pharmacology of the Renin-Angiotensin System," Drugs 39 (Suppl. 1):21-31 (1990).
Blair-West, J.R. et al., "Effect of the Heptapeptide (2-8) and Hexapeptide (3-8) Fragments Angiotensin II on Aldosterone Secretion," J. Clin. Endocrinol. Metab. 32:575-578 (1971).
Harding, J.W. et al., "Angiotensin-Sensitive Neurons in the Rat Paraventricular Nucleus: Relative Potencies of Angiotensin II and Angiogensin III," Brain Res. 410:130-134 (1987).
Regoli, D. et al., "The Enzymatic Degradation of Various Angiotensin II Derivatives By Serum, Plasma or Kidney Homogenate," Biochem. Pharmacol. 12:637-646 (1963).
Bumpus, F.M. et al., "The Relationship of Structure to Pressor and Oxytocic Actions of Isoleucine.sup.5 Angiotensin Octapeptide and Various Analogues," Biochim. Biophys. Acta 46:38-44 (1961).
Regoli, D. et al., "Pharmacology of Angiotensin," Pharmacol. Reviews 26:69-123 (1974).
Bennett, J.P. et al., "Angiotensin II Binding to Mammalian Brain Membranes," J. Biol. Chem. 251:7423-7430 (1976).
Glossman, H. et al., "Properties of Angiotensin II Receptors in the Bovine and Rat Adrenal Cortex," J. Biol. Chem. 249:825-834 (1974).
Fitzsimons, J.T., "The Effect on Drinking of Peptide Precursors and of Shorter Chain Peptide Fragments of Angiotensin II Injected into the Rat's Diencephalon," J. Physiol. Lond. 214:295 (1971).
Tonnaer, V.M., "Central Effects of Angiotensins on Drinking and Blood Pressure: Structure-Activity Relationships," Brain Res. 236:417 (1982).
Siemens, I.R. et al., "Solubilization and Partial Characterization of Angiotensin II Receptors from Rat Brain," J. Neurochem 57:690-700 (1991).
Kono, T. et al., "Biological Activity of Des-(Asp.sup.1, Arg.sup.2, Val.sup.3)-Angiotensin II in Man," Life Sci. 32:337-343 (1983).
Kono, T. et al., "Responses of Patients with Bartter's Syndrome to Angiotensin II and Angiotensin II-(3-8)-Hexapeptide," Acta Endocr. 109:249-253 (1985).
Haberl, R.L. et al., "Angiotensin Degradation Products Mediate Endothelium-Dependent Dilation of Rabbit Brain Arterioles," Circ. Res. 68:1621-1627 (1991).
Braszko, J.J. et al., "The 3-7 Fragment of Angiotensin II is Probably Responsible For its Psychoactive Properties," Brain Res., 542:49-54 (1991).
Braszko, J.J. et al., "Angiotensin II-(3-8)-Hexapeptide Affects Motor Activity, Performance of Passive Avoidance and a Conditioned Avoidance Response in Rats," Neurosci. 27:777-783 (1988).
Barszko, J.J. et al., "Psychotropic Effects of Angiotensin II and III in Rats: Locomotor and Exploratory vs. Cognitive Behavior," Behav. Brain. Res. 25:195-203 (1987).
Semple, P.F. et al., "Angiotensin II and its Heptapeptide (2-8), Hexapeptide (3-8), and Pentapeptide (4-8) Metabolites in Arterial and Venous Blood of Man," Circ. Res. 39:671-678 (1976).
Blumberg, A.L. et al., "Angiotensin (A I, A II, A III) Receptor Characterization," Circ. Res. 41:154-158 (1977).
Bennett, J.P. et al., "Receptor Binding Interactions of the Angiotensin II Antagonist, .sup.125 I-[Sarcosine.sup.1,Leucine.sup.8 ] Angiotensin II, With Mammalian Brain and Peripheral Tissues," Eur. J. Pharmacol. 67:11-25 (1980).
Fernandez, L.A. et al., "Neovascularization Produced by Angiotensin II," Lab. Clin. Med. 105:141-145 (1985).
Patel, J.M. et al., "Angiotensin Receptors in Pulmonary Arterial and Aortic Endothelial Cells," Am. J . Physiol. 256:C987-C993 (1989).
Baker, K.M. et al., "Angiotensin II Stimulation of Protein Synthesis and Cell Growth in Chick Heart Cells," Am. J. Physiol. 259:H610-H618 (1990).
Baker, K.M. et al., "Renin-Angiotensin System Involvement in Pressure-Overload Cardiac Hypertrophy in Rats," Am. J. Physiol. 259:H324-H332 (1990).
Yamaguchi, T. et al., "Role of the Adrenal Renin-Angiotensin System on Adrenocorticotropic Hormone- and Potassium-Stimulated Aldosterone Production by Rat Adrenal Glomerulosa Cells in Monolayer Culture," Hypertension 16:635-641 (1990).
Carpenter, G. et al., "Rapid Enhancement of Protein Phosphorylation in A-431 Cell Membrane Preparations by Epidermal Growth Factor," J. Biol. Chem. 254:4884-4891 (1979).
Munson, P.J. et al., "Ligand: A Versatile Computerized Approach for Characterization of Ligand-Binding Systems," Anal. Biochem. 107:220-239 (1980).
Freissmuth, M. et al., "G Proteins Control Diverse Pathways of Transmembrane Signaling," FASEB J. 3:2125-2131 (1989).
Brown, A.M. et al., "Direct G Protein Gating of Ion Channels," Am. J. Physiol. 254:H401-410 (1988).
Schulz, S. et al., "The Guanylate Cyclase/Receptor Family of Proteins," FASEB J. 3:2026-2035 (1989).
Nishibe, S. et al., "Increase of the Catalytic Activity of Phospholipase C.gamma.1 by Tyrosine Phosphorylation," Science 250:1253-1256 (1990).
Pandiella, A. et al., "Transmembrane Signalling at the Epidermal Growth Factor Receptor," TiPS 10:411-414 (1989).
Cohen, S. et al., "Epidermal Growth Factor-Receptor-Protein Kinase Interactions," J. Biol. Chem. 255:4834-4842 (1980).
Pang, D.T. et al., Protein Tyrosine Phosphorylation in Synaptic Vesicles, Proc. Nat. Acad. Sci. USA 85:762-766 (1988).
Wright, J.W. et al., "Structure-Function Analyses of Brain Angiotensin Control of Pressor Action in Rats," Am. J. Physiol. 257:R1551-R1557 (1989).
Gill, G.N. et al., "Angiotensin Stimulation of Bovine Adrenocortical Cell Growth," Proc. Nat. Acad. Sci. USA 74:5569-5573 (1977).
Aceto, J.F. et al., "[Sar.sup.1 ]Angiotensin II Receptor-Mediated Stimulation of Protein Synthesis in Chick Heart Cells," Am. J. Physiol. 258:H806-H813 (1990).
Mendelsohn, F.A.O. et al., "Autoradiographic Localization of Angiotensin II Receptors in Rat Brain," Proc. Natl. Acad. Sci. USA 81:1575-1579 (1984).
Harding, J.W. et al., "Angiotensin and Blood Pressure Regulation," Acad. Press, San Diego, CA. p. 1-34 (1988).
Paul, A.K. et al., "Coexistence of Guanylate Cyclase and Atrial Natriuretic Factor Receptor in 180-kD Protein," Science 235:1224-1226 (1987).
Abhold, R.H. et al., "Metabolism of Angiotensins II and III by Membrane-Bound Peptidases from Rat Brain," J. Pharmacol Exp. Ther. 245:171-177 (1988).
International Search Report, PCT/US93/06038.
Sardinia, M.F. et al., "AT.sub.4 Receptor Binding Characteristics: D-Amino Acid- and Glycine-Substituted Peptides," Peptides 14:949-954 (1993).
Hanesworth, J.M. et al., "Elucidation of a Specific Binding Site for Angiotensin II(3-8), Angiotensin IV, in Mammalian Heart Membranes," The Journal of Pharmacology and Experimental Therapeutics 266(2):1036-1042 (1993).
Swanson, G.N. et al., "Discovery of a distinc
Harding Joseph W.
Wright John W.
Hamud Fozia
Mertz Prema
Washington State University Research Foundation
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