Chemistry: analytical and immunological testing – Heterocyclic carbon compound
Patent
1996-02-01
1999-11-30
MacMillan, Keith D.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
436 92, 436546, 436106, 436119, 436800, 8648, 548100, 548416, 548452, 548469, 548471, 548513, 549 1, 549 6, 549 7, G01N 3300, G01N 33533, C09B 500
Patent
active
059941430
ABSTRACT:
Fluorescent conjugates suitable for use in flow cytometry and other biological applications. The fluorescent conjugates comprise an antibody having a polymeric dye bound thereto. The polymeric dye is preferably enhanced by a hydrophobic and conformationally restrictive moiety either bound thereto or in close association therewith. The hydrophobic and conformationally restrictive moiety is preferably derived from a cyclodextrin. The polymeric dye comprises a polymeric entity having signal-generating groups, such as aminostyryl pyridinium dye residues attached thereto. The fluorescent conjugates exhibit exceptional stability characteristics and avoid many of the problems of energy transfer, bio-conjugability, and solubility.
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Bieniarz Christopher
Cornwell Michael J.
Huff Jeffrey B.
Tata Venkata Seshagiri R.
Abbott Laboratories
MacMillan Keith D.
Ponnalun P.
Weinstein David L.
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