Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-08-26
1999-11-30
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 723, 435 792, 435962, 436174, 436175, 436177, 436178, 436518, 436536, 436538, 436539, 436540, 436541, 436 64, 436811, 436813, 436824, 436825, G01N 33574, G01N 33537, G01N 33541
Patent
active
059940859
ABSTRACT:
The present invention relates to novel methods and devices for detecting non-complexed prostate specific antigen (free PSA), which is used in conjunction with total PSA tests to identify patients having either benign prostatic diseases (BPD), such as benign prostatic hyperplasia, prostatitis, or glandular atrophy or prostatic adenocarcinoma (CAP). In a biological sample, one can find not only free PSA, but also prsotate specific antigen (PSA) which has formed a complex with .alpha.1-antichymotrypsin (ACT). The present invention removes complexed PSA (PSA-ACT) from a fluid sample, thereby removing any possible interference due to binding of complexed PSA to an allegedly free PSA specific antibody in an immunoassay for free PSA. The method requires contacting a biological fluid sample possibly containing a mixture of complexed PSA and free PSA with a device which specifically binds only complexed PSA, leaving any free PSA unbound, prior to exposing the sample to assay reagents and conditions for detecting free PSA in a specific binding reaction. The device can be a filter having a modified filter media that permits the sample to flow or be pulled through the filter media and into assay reagents. Alternatively, the device can be a removable media that is placed into a sample, allowed to remain long enough to bind any complexed PSA present, and then withdrawn from the sample prior to running a free PSA specific binding assay.
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Chin Christopher L.
Voyce Brian D.
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