Methods for identifying a mutation in a gene of interest without

Details Methods for identifying a mutation in a gene of interest without Methods for identifying a mutation in a gene of interest without

1997-05-16

1999-11-30

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435441, 435444, 435446, C12Q 168, C12P 1934

Patent

active

059940751

ABSTRACT:
The invention encompasses methods of identifying a mutation in a gene of interest in an organism which include identifying in a test DNA sample from a mutated organism a mutation in a gene of interest without the prior observation of a phenotypic alteration in the mutated organism.

REFERENCES:
patent: 5045450 (1991-09-01), Thilly et al.
patent: 5190856 (1993-03-01), Borresen
patent: 5347075 (1994-09-01), Sorge
patent: 5429923 (1995-07-01), Seidman et al.
patent: 5510099 (1996-04-01), Short et al.
Kaneko et al., Laboratory Animals 29(4), 442-446 (Oct. 1995).
Gordon et al., Mutation Research 243, 145-149 (1990).
Ashburner, 1989, "N-Ethyl-N-Nitrosourea", in Drosophila, A Laboratory Handbook, Ch. 9, section 3.2.3, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Bode et al., 1988, hph-1: a mouse mutant with hereditary hyperphenylalaninemia induced by ethylnitorsourea mutagenesis, Genetics, 118: 299-305.
Casadaban and Cohen, 1979, Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: In vivo probe for transcriptional control sequences, Proc. Natl. Acad. Sci. U.S.A., 76: 4530-4533.
Chiocca et al., 1992, Genotypic analysis of N-ethyl-N-nitrosourea-induced mutations by Taq I restriction fragment length polymorphism/polymerase chain reaction in c-H-ras1 gene, Proc. Natl. Acad. Sci. U.S.A., 89: 5331-5335.
Colledge et al., 1995, Generation and characterization of a delta F508 cytic fibrosis mouse model, Nature Genetics, 10: 445-452.
Ellison et al., 1993, Efficacy of fluorescence-based PCR-SSCP for detection of point mutations, Biotechniques, 15: 684-691.
Faham and Cox, 1995, A novel in vivo method to detect DNA sequence variation, Genome Res., 5: 474-482.
Favor, 1986, The frequency of dominanat cataract and recessive specific-locus mutations in mice derived from 80 or 160 mg ethylnitrosourea per kg body weight treated spermatogonia, Mutat. Res., 162(1): 69-80.
Glavac and Dean, 1993, Optimization of the single-strand information polymorphism (SSCP) technique for detection of point mutations, Hum. Mutat., 2: 404-414.
Harding et al., 1992 Sar: A genetic mouse model for human sarcosinemia generated by ethylnitrosourea mutagenesis, Proc. Natl. Acad. Sci. U.S.A., 89: 2644-2648.
Johnson and Lewis, 1981, Electrophoretically detected germinal mutations induced in the mouse by ethylnitrosourea, Proc. Natl. Acad. Sci. U.S.A., 78(5): 3138-3141.
Khrapko et al., 1994, Constant denaturan capillary electrophoresis (CDCE): a high resolution approach to mutational analysis, Nucleic Acids Res., 23(3): 364-369.
Lewis et al., 1985, A mutation in the .beta.-globin gene detected in the progeny of a female mouse treated with ethylnitrosourea, Proc. Natl. Acad. Sci. U.S.A., 82(17): 5829-5831.
McWhir et al., 1993, Mice with DNA repair gene (ERCC-1) deficiency have elevated levels of p53, liver nuclear abnormalities and die before weaning, Nature Genetics, 5: 217-224.
Mullins et al., 1994 Large-scale mutagenesis in the zebrafish: in search of genes controlling development in a vertabrae, Curr. Biol., 4: 189-202.
Palombo et al., 1992, Non-phenotypic selection of N-methyl-N-nitrosourea-induced mutations in human cells, Nucleic Acids Res.., 20: 1349-1354.
Rossant et al., 1992, Of fin and fur: mutational analysis of vertebrate embryonic development, Genes Dev., 6: 1-13.
Russell et al., 1979, Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse, Proc. Natl. Acad. Sci. U.S.A., 76: 5818-5819.
Russell et al., 1982, Dose-response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia, Proc. Natl. Acad. Sci. U.S.A., 79: 3589-3591.
Russell et al., 1989, Chlorambucil effectively induces deletion mutations in mouse germ cells, Proc. Natl. Acad. Sci. U.S.A., 86: 3704-3708.
Sadlack et al., 1993, Ulcerative colitis-like disease in mice with a disruptive interleukin-2 gene, Cell, 75: 253-261.
Sheffield et al., 1993, The sensitivity of single-strand conformation polymorphism analysis for hte detection of single base substitution, Genomics, 16: 325-332.
Solnica-Krezel et al., 1994, Efficient recovery of ENU-induced mutations from the zebrafish germline, Genetics, 136: 1401-1420.
Stevanovic et al., 1994, The cDNA sequence and chromosomal location of the human SOX2 gene, Mammalian Genome, 5: 640-642.
Yuan et al., 1995, Development-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3, Genes Dev., 9: 2635-2645.

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