Homogeneous methods for nucleic acid amplification and detection

Details Homogeneous methods for nucleic acid amplification and detection Homogeneous methods for nucleic acid amplification and detection

1991-05-02

1999-11-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435810, 436 63, 436 94, C12P 148, C12P 168, C12N 1510

Patent

active

059940565

ABSTRACT:
This invention relates to improved methods for nucleic acid detection using methods such as the polymerase chain reaction (PCR). More specifically, the invention provides methods for simultaneous amplification and detection to enhance the speed and accuracy of prior methods. The methods involve the introduction of detectable DNA binding agents into the amplification reaction, which agents produce a detectable signal that is enhanced upon binding double-stranded DNA. In a preferred embodiment, the binding agent is a fluorescent dye. The methods also provide means for monitoring the increase in product DNA during an amplification reaction.

REFERENCES:
patent: 4119521 (1978-10-01), Chirikjian
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5049490 (1991-09-01), Sutherland et al.
Kirk-Othmer, "Antibiotics (Phenazines) to Bleaching Agents," Encyclopedia of Chemical Technology (Third Edition), 3:906-907.
Byrnes, J.J., et al., "Bone Marrow Cytoplasmic Deoxyribonucleic Acid polymerase, Variation of pH and Ionic Environment as a Possible Control Mechanism," Biochemistry, 12/22:4378-4384 (1973).
Lomell, H., et al., "Quantitative Assays Based on the use of Replicatable Hybridization Probes," Clin. Chem., 35/9:1826-1831 (1989).
Isaacs et al., Jan., 1991, "Post-PCR Sterilization: Development and Application to an HIV-1 Diagnostic Assay" Nucleic Acids Research 19(1):109-116 (1991).
Share et al., 1973, Biochemistry 12:3055.
Morrison et al., 1989, Anal. Biochem. 183:231-244.
Glazer et al., 1990. Proc. Natl. Acad. Sci. USA 87:3851-3855.
Mabuchi and Nishikawa, 1990, Nuc. Acids Res. 18(24):7461-7462.
Oser and Valet, 1990, Angew. Chem. Int. Engl. 29(10):1167.
Sobel and Jair 1972, J. Mol. Biol. 68:21-34.
Kapuseinski and Szer, 1979, Nuc. Acids Res. 6(112):3519-3535.
Johnson and Hearst, 1981, Photochem. and Photobiol. 33:785-791.
Khattar et al., 1990, JACS 112:4960.
Searle and Embrey, 1990, Nuc. Acids Res. 18(13):3653-3762.
Richardson, 1973, J. Mol. Biol. 78:703-714.
Kornberg, 1974, in DNA Synthesis, W.H. Freman and Co., San Francisco, pp. 220-236.

Affiliated with

Also associated with

Rating

Homogeneous methods for nucleic acid amplification and detection does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Homogeneous methods for nucleic acid amplification and detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homogeneous methods for nucleic acid amplification and detection will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1669915