Nucleic acid encoding synthesis-deficient thermostable DNA polym

Details Nucleic acid encoding synthesis-deficient thermostable DNA polym Nucleic acid encoding synthesis-deficient thermostable DNA polym

1993-06-04

1996-07-30

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4353201, 4352528, C12N 121, C12N 1500, C07H 2100, C07H 2104

Patent

active

055413110

ABSTRACT:
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. Cleaving enzymes are produced through the use of novel DNA sequences which encode novel thermostable polymerases.

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M. Longl

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