Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1986-06-06
1989-03-28
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
422 61, 435 7, 435 28, 435239, 435805, 435810, C12Q 170
Patent
active
048163872
ABSTRACT:
The invention relates to a rapid and sensitive assay method for the detection of antibodies to Human T-Cell Leukemia Virus-III (HTLV-III), the AIDS virus, and diagnostic test kits for carrying out the method of the invention. According to the method of the invention, which is referred to as a Quick Western Blot, test samples and viral lysate are used in at least 50% greater concentrations than those used in conventional Western Blot techniques. In accordance with the invention, test results may be obtained in under two hours.
REFERENCES:
patent: 4452901 (1984-06-01), Gordon et al.
patent: 4520113 (1985-05-01), Gallo et al.
patent: 4629783 (1986-12-01), Cosand
patent: 4661445 (1987-04-01), Saxinger et al.
R. Gallo et al., Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDS, Science 224, 500-503, May 4, 1984.
J. Carlson et al., AIDS Serology Testing in Low-and High-Risk Groups, JAMA 253, 3405-3408, Jun. 21, 1985.
S. Weiss et al., Screening Test for HTLV-III (AIDS Agent) Antibodies, JAMA 253, 221-225, Jan. 11, 1985.
J. Schupbach et al., Antibodies to HTLV-III in Swiss Patients with AIDS and pre-AIDS in Groups at Risk for AIDS, N. Engl. J. Med. 312, 265-270, Jan. 31, 1985.
G. Biberfeld et al., Antibodies to Human T Lymphotropic Virus Type III Demonstrated by a Dot Immunobinding Assay, Scand. J. Immunol. 21, 289-292, 1985.
V. Tsang et al., Enzyme-Linked Immunoelectrotransfer Blot Techniques (EITB) for Studying the Specificities of Antigens and Antibodies Separated by Gel Electrophoresis, Method in Enzymology 92, 377-391, 1983 Academic Press.
Towbin et al., "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications" Proc. Nat'l Acad. Sci. U.S.A. 76:4350-4354 (1979).
Groopman et al., "Serological Characterization of HTLV-III Infection in AIDS and Related Disorders" J. Infec. Dis. 153:736-742 (Apr. 1986).
Geroldi et al., "Western Blot Technique in the Serological Evaluation of Three LAV/HTLV III-infected Italian Families" Infection: 60-63 (1986).
Safai et al., "Seroepidemiological Studies of Human T-Lymphotropic Retrovirus Type III in Aquired Immunodeficiency Syndrome" Lancet I: 1438-1440 (Jun. 30, 1984).
Cabradilla et al., "Serodiagnosis of Antibodies to the Human AIDS Retrovirus with a Bacterially Synthesized env Polypeptide" Bio/Technology 4:128-133 (Feb. 1986).
Bio-Research Laboratories, Inc.
Nucker Christine M.
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