Non-radioactive method for detecting a labelled segment and a so

Details Non-radioactive method for detecting a labelled segment and a so Non-radioactive method for detecting a labelled segment and a so

1993-01-28

1994-10-11

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 794, 435 21, C12Q 168, G01N 3353

Patent

active

053546580

ABSTRACT:
A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change indicative of the presence of the labelled complex. A solution or composition of bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT), as well as a test kit including the same, is also provided for carrying out the chromogenic method of detection.

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Heegaard, Applied and Theoreti. Electropho. 1:261-2364 (1990) "Visualization of Alkaline Phosphase". . . .
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Blake et al., Anal. Biochem 136:175-179 (1984) "A Rapid, Sensitive Method for Detection".

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