Process for the highly specific detection of nucleic acids in so

Details Process for the highly specific detection of nucleic acids in so Process for the highly specific detection of nucleic acids in so

1992-12-18

1994-10-11

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, 935 77, 935 78, C12Q 168, C07H 2104, C12N 1500

Patent

active

053546571

ABSTRACT:
The present invention provides a process for the detection of nucleic acids of definite sequence by hybridisation with two single-stranded nucleic acid probes present in the same solution phase complementary to different regions of the nucleic acid to be detected, one nucleic acid probe serving as detector probe and containing as labelling at least one hapten bound via a chemical linkage and the other nucleic acid probe serving as capturing probe and being bound to a solid matrix, wherein, as hapten, a steroid is used which on at least one position of the detector probe, which does not participate in the hydrogen bridge formation with the nucleic acid to be detected, is covalently bound via a bridge of at least four atoms, the nucleic acid to be detected, present in solution, is incubated with the detector probe and the capturing probe in any desired sequence, whereby the binding of the capturing probe on the matrix with the nucleic acid to be detected is brought about before, during or after the incubation, the remaining solution is separated off from the matrix-bound complex of the capturing probe, nucleic acid to be detected and detector probe and the complex detected via an anti-hapten antibody which is labelled.

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