Assay having improved dose response curve

Details Assay having improved dose response curve Assay having improved dose response curve

1992-02-28

1996-07-30

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435188, 435962, 436518, 436 63, 436 537, 436540, 422 57, 422 58, 422100, 422101, G01N 33535, G01N 33536, G01N 33538, G01N 3374

Patent

active

055410693

ABSTRACT:
An assay for determining the presence of a threshold concentration of an analyte in a sample comprises first reacting the sample with an amount of anti-analyte selected to reduce the free analyte to a marginally detectable concentration. The sample is then contacted with anti-analyte immobilized on a test region or indicator zone on a solid phase, whereby the residual free analyte may be bound. The binding of free analyte to immobilized anti-analyte is detected by a variety of techniques to indicate the presence of the threshold concentration. By employing limited amounts of anti-analyte on the solid phase, the change between maximum binding of label and no binding of label will be responsive to very small changes in the analyte concentration originally present in the sample.

REFERENCES:
patent: 4818677 (1989-04-01), Hay-Kaufman et al.
patent: 4920046 (1990-04-01), McFarland et al.
patent: 4943522 (1990-07-01), Eisinger et al.
patent: 4952517 (1990-08-01), Bahar
patent: 5028535 (1991-07-01), Buechler et al.
patent: 5079170 (1992-01-01), Rosman et al.
D. Catty et al "ELISA and Related Enzyme Immunoassays" in Antibodies vol. II a Practical Approach Ed. D. Catty 1989 IRL Press pp. 97-154.

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