Methods and compositions for preparing protein processing enzyme

Details Methods and compositions for preparing protein processing enzyme Methods and compositions for preparing protein processing enzyme

1993-07-28

1996-07-30

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352402, 4353201, 536 235, C12Q 168, C12N 510, C12N 1511, C07H 2100

Patent

active

055410626

ABSTRACT:
The present invention relates to a new protein, convertin I, which belongs to a family of mammalian convertase proteins characterized by partial amino acid sequence homology to each of the following: the precursor processing endoprotease of yeast KEX2, bacterial subtilisins, and the human fur gene product, furin. The protein which is a subject of this invention is further characterized as a mammalian convertase, which is capable of hydrolytically cleaving, for example, prohormones to yield active hormones. Specifically, convertin I is capable of cleaving peptide bonds following certain sequences of two adjacent basic amino acids. Convertin I has a molecular weight of approximately 70 kd by electrophoresis. Another aspect of this invention is a recombinant vector, incorporating the coding sequences which are the objects of this invention. Methods of preparing the endoprotease protein, including molecular engineering methods are described. This invention also relates to a nucleic acid segment, including DNA and a cDNA having a sequence coding for convertin I. Methods of detection of said protein by, for example, fluorescent antibodies, and use of its coding sequences as hybridization probes are also provided.

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