Multidrug resistance in mammalian cell lines and isolation of de

Details Multidrug resistance in mammalian cell lines and isolation of de Multidrug resistance in mammalian cell lines and isolation of de

1985-09-10

1990-03-27

Tarcza, John Edward

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 91, 435320, 43525233, 4351723, 935 11, 935 12, 536 27, C12P 2100, C12P 1934, C12N 1500, C12N 120

Patent

active

049120395

ABSTRACT:
A method for isolating a cDNA specific for P-glycoprotein is disclosed. The P-glycoprotein is the determinant in the multidrug resistance phenotype exhibited by living cells. The nucleic acid equivalent of the cDNA may be cloned in a recombinant plasmid or phage. The cDNA may also be used as a probe in determining multidrug resistance in cells. The DNA molecules specific for the P-glycoprotein is in the form of a gene family where various members of the gene family are amplified to varying degrees in multidrug resistant cells from various sources.

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Roninson et al., "Amplification of Specific DNA Sequences Correlates with Multi-Drug Resistance in Chinese Hamster Cells", Nature, vol. 309, pp. 626-628 (1984).

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