Method for purifying nucleic acids from heterogenous mixtures

Details Method for purifying nucleic acids from heterogenous mixtures Method for purifying nucleic acids from heterogenous mixtures

1995-09-15

1998-07-21

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2541, 536 2542, 536127, 435803, 423335, C07H 100

Patent

active

057836868

ABSTRACT:
Discloses DNA isolation and purification methods which involve novel washing steps. The disclosed methods provide a means for isolating and purifying DNA from a homogeneous mixture of DNA of other cellular contaminants by treating silica with the homogeneous mixture containing DNA in the presence of a chaotropic salt solution and then washing and separating the washed and treated silica in successive wash steps with aqueous alcohol wash solutions. A first wash step involves washing the treated silica with a first wash solution of at least 95 wt % alcohol in water. A second wash step similarly involves washing the treated and washed silica with second wash solution of less than 95 wt % alcohol in water.

REFERENCES:
patent: 4923978 (1990-05-01), McCormich
patent: 5057426 (1991-10-01), Henco et al.
patent: 5075430 (1991-12-01), Little
patent: 5155018 (1992-10-01), Gillespie et al.
patent: 5204257 (1993-04-01), DeBonville et al.
patent: 5273718 (1993-12-01), Skold et al.

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