Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Metal containing – e.g. – chromoproteins – ferritin,...
Patent
1996-10-10
1998-07-21
Achutamurthy, Ponnathapura
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Metal containing, e.g., chromoproteins, ferritin,...
530370, 530395, 530345, 530807, 530403, 530404, 530405, 530408, 436501, 436536, 436546, 435961, 435964, A61K 3578, A61K 3580, C07K 1700, G01N 33531
Patent
active
057836736
ABSTRACT:
A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.
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Achutamurthy Ponnathapura
Coulter Corporation
Kaye Michelle A.
Ponnaluri P.
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